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American Society of Microbiology ABMM Sample Questions:

1. A patient with HIV infection and a very low CD4+ T-cell count develops a disseminated infection with a fungus that is commonly found in pigeon droppings. The MOST likely causative agent is:

A) Candida glabrata
B) Saccharomyces cerevisiae
C) Cryptococcus gattii
D) Cryptococcus neoformans

2. During the processing of stool specimens for ova and parasite examination, the formalin-ethyl acetate concentration procedure primarily serves to:

A) Stain parasite nuclei for easier identification
B) Selectively culture protozoan trophozoites
C) Preserve parasite morphology and kill bacteria
D) Separate parasites from fecal debris and concentrate them

3. A patient develops a severe diarrheal illness after consuming raw seafood. Stool culture yields a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing blue-green colonies. The MOST significant virulence factor contributing to the severe, cholera-like diarrhea caused by this organism is:

A) Type III secretion system (T3SS)
B) Heat-labile toxin (LT)
C) Heat-stable enterotoxin (ST)
D) Cholera toxin (CT)-like enterotoxin

4. A patient undergoing chemotherapy for leukemia develops a severe pneumonia. Bronchoalveolar lavage fluid is examined, revealing numerous cysts containing crescent-shaped structures upon silver staining. The patient's CD4+ T-cell count is significantly low. The MOST likely diagnosis is infection with:

A) Histoplasma capsulatum
B) Coccidioides immitis
C) Pneumocystis jirovecii
D) Blastomyces dermatitidis

5. A patient with a history of inflammatory bowel disease is diagnosed with primary sclerosing cholangitis and undergoes liver transplantation. Post-transplant, they develop a bloodstream infection with a Gram-negative bacterium that is identified as Fusobacterium nucleatum. This organism's role in the pathogenesis of colorectal cancer is primarily attributed to its ability to:

A) Produce potent exotoxins that damage the intestinal epithelium.
B) Form biofilms that physically obstruct the bile ducts.
C) Modulate the host immune response and promote a pro-inflammatory microenvironment.
D) Directly induce mutations in colonic epithelial cells.

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